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pwpi-ires-bla-ak-ace2-tmprss2  (Addgene inc)


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    Addgene inc pwpi-ires-bla-ak-ace2-tmprss2
    Pwpi Ires Bla Ak Ace2 Tmprss2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pwpi-ires-bla-ak-ace2-tmprss2/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    pwpi-ires-bla-ak-ace2-tmprss2 - by Bioz Stars, 2026-03
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    Rheb1 promotes β cell proliferation only partly depend on the mTORC1. A: The population of 5-ethy-nyl-2’-deoxyuridine + cells of MIN6 cells treated with PWPI or Rheb1OE lentivirus with or without rapamycin treatment; B: The population of Ki67+ cells of insulin+ islet treated with GFP or Rheb1OE adenovirus with or without rapamycin treatment; C: Western blot analysis of C57 mice islets treated with GFP or Rheb1OE adenovirus for 48 hours with or without rapamycin treatment ( n = 3/group). All data are represented as the mean ± SD, a P < 0.05, b P < 0.001. NC: Negative control; Edu: 5-ethy-nyl-2’-deoxyuridine.

    Journal: World Journal of Diabetes

    Article Title: Ras homolog enriched in brain 1 regulates β cell mass and β cell function via mTORC1/AMPK/Notch1 pathways

    doi: 10.4239/wjd.v16.i6.104973

    Figure Lengend Snippet: Rheb1 promotes β cell proliferation only partly depend on the mTORC1. A: The population of 5-ethy-nyl-2’-deoxyuridine + cells of MIN6 cells treated with PWPI or Rheb1OE lentivirus with or without rapamycin treatment; B: The population of Ki67+ cells of insulin+ islet treated with GFP or Rheb1OE adenovirus with or without rapamycin treatment; C: Western blot analysis of C57 mice islets treated with GFP or Rheb1OE adenovirus for 48 hours with or without rapamycin treatment ( n = 3/group). All data are represented as the mean ± SD, a P < 0.05, b P < 0.001. NC: Negative control; Edu: 5-ethy-nyl-2’-deoxyuridine.

    Article Snippet: The Rheb1 OE MIN6 cell line and its corresponding control were developed as follows: Full-length mouse Rheb1 cDNA (amino acids 1-185) was inserted into the PWPI plasmid from Addgene, which contains a C-terminal FLAG tag.

    Techniques: Western Blot, Negative Control